2PC5
Native crystal structure analysis on Arabidopsis dUTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.903, 70.860, 75.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.460 - 2.200 |
| R-factor | 0.18508 |
| Rwork | 0.182 |
| R-free | 0.25075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q5h |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.075 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.123 |
| Number of reflections | 19648 | |
| <I/σ(I)> | 57.1 | 37.9 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 0.2 M ammonium sulfate, 0.1 M Tris-HCl pH 9.0, 0.02 M taurine, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
