2PBO
E27Q mutant of EXO-B-(1,3)-Glucanase from Candida Albicans at 1.85 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.000, 65.170, 96.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.440 - 1.850 |
R-factor | 0.131 |
Rwork | 0.130 |
R-free | 0.15800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eqp |
RMSD bond length | 0.011 |
RMSD bond angle | 1.231 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.040 | 0.025 | 0.144 |
Number of reflections | 32687 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 91.1 | 81.7 | 91.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | PEG 8000, Hepes, CaCl2, pH 7.3, temperature 293K, VAPOR DIFFUSION, HANGING DROP |