2PB7
Crystal Structure of the SRA domain of the human UHRF1 protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8730, 0.9791, 0.9795, 0.9757 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.929, 53.929, 161.995 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| Starting model (for MR) | MAD model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.375 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 22424 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 94.0 | 95.6 |
| Redundancy | 5.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 14-19% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
