2PAH

TETRAMERIC HUMAN PHENYLALANINE HYDROXYLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-07
Detector	MARRESEARCH
Spacegroup name	P 31 1 2
Unit cell lengths	119.500, 119.500, 126.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.100
R-factor	0.251
Rwork	0.251
R-free	0.32600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1toh
RMSD bond length	0.006
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.075	0.250
Total number of observations	120922 *
Number of reflections	18446 *
<I/σ(I)>	10	2
Completeness [%]	99.0	98
Redundancy	6.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		0.6 M MGSO4, 0.2 M NAK TARTRATE, 10% PEG-4000, 0.1 M BIS-TRIS PROPANE (PH 7.0)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.6 (M)
2	1	reservoir	sodium potassium tartrate	0.2 (M)
3	1	reservoir	PEG4000	10 (%)
4	1	reservoir	Bis-Tris propane	100 (mM)