2P9A

E. coli methionine aminopeptidase dimetalated with inhibitor YE6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	37.986, 60.649, 50.486
Unit cell angles	90.00, 104.82, 90.00

Refinement procedure

Resolution	20.000 - 1.600
Rwork	0.206
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.295
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	18.850	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge	0.033	0.027	0.220
Total number of observations		3337	14214
Number of reflections	28157
<I/σ(I)>	15.2	19.4	3.4
Completeness [%]	96.3	97.6	93.5
Redundancy	3.7	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K