2P8E
Crystal structure of the serine/threonine phosphatase domain of human PPM1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.399, 92.315, 118.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.816 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a6q |
RMSD bond length | 0.015 |
RMSD bond angle | 1.503 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.932 | 1.910 |
High resolution limit [Å] | 1.816 | 1.816 |
Rmerge | 0.184 | 0.842 |
Number of reflections | 49509 | |
<I/σ(I)> | 8.3 | 1.5 |
Completeness [%] | 93.8 | 84.5 |
Redundancy | 5.4 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Hepes pH 7.5, 20% PEG 4000, 10% Isopropanol, VAPOR DIFFUSION, temperature 294K |