2P7T

Crystal Structure of KcsA mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-13
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	156.339, 156.339, 76.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.050
Rwork	0.219
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.285
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.050	2.050
Rmerge	0.050
Number of reflections	55013
<I/σ(I)>	33
Completeness [%]	95.6	90
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	pH 5.2-5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K