2P6T
CRYSTAL STRUCTURE OF TRANSCRIPTIONAL REGULATOR NMB0573 and L-LEUCINE COMPLEX FROM NEISSERIA MENINGITIDIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.389, 149.771, 77.845 |
| Unit cell angles | 90.00, 105.68, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.23853 |
| Rwork | 0.235 |
| R-free | 0.30008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p5v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.054 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.143 | 0.386 |
| Number of reflections | 28887 | |
| <I/σ(I)> | 6.3 | 1.3 |
| Completeness [%] | 91.2 | 63.2 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 95 mM HEPES-Na, 190 mM Calcium Chloride, 26.6% PEG 400, 5% Glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
