2P5T
Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-08-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.520, 102.860, 254.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 3.200 |
| R-factor | 0.21692 |
| Rwork | 0.214 |
| R-free | 0.27732 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.060 | 0.387 |
| Number of reflections | 34352 | |
| <I/σ(I)> | 20.8 | 3.1 |
| Completeness [%] | 95.3 | 85.5 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 12.5-15% (v/v) iso-propanol, 100 mM MES-NaOH, 6% (v/v) dioxane (30% (v/v)), pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
