2P5O
Crystal structure of RB69 GP43 in complex with DNA containing an abasic site analog
Replaces: 1RV2Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-05 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9500, 0.9788, 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.018, 123.209, 165.616 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.800 |
| Rwork | 0.245 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.307 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 234220 | |
| Completeness [%] | 90.9 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 8% PEG 2000 MME. 100mM Magnesium sulfate, 100mM HEPES, 100mM Sodium acetate, 15% glycerol, 2mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 | ||
| 10 | 1 | 2 | HEPES | ||
| 11 | 1 | 2 | glycerol | ||
| 2 | 1 | 1 | Magnesium sulfate | ||
| 3 | 1 | 1 | Sodium acetate | ||
| 4 | 1 | 1 | HEPES | ||
| 5 | 1 | 1 | glycerol | ||
| 6 | 1 | 1 | BME | ||
| 7 | 1 | 2 | PEG 2000 | ||
| 8 | 1 | 2 | Magnesium sulfate | ||
| 9 | 1 | 2 | Sodium acetate |
