2P5E
Crystal Structures of High Affinity Human T-Cell Receptors Bound to pMHC Reveal Native Diagonal Binding Geometry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-05 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.002, 51.771, 118.270 |
| Unit cell angles | 90.00, 98.24, 90.00 |
Refinement procedure
| Resolution | 117.040 - 1.890 |
| R-factor | 0.18071 |
| Rwork | 0.178 |
| R-free | 0.23579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f53 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.173 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 117.000 |
| High resolution limit [Å] | 1.887 |
| Number of reflections | 69966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 85 mM HEPES, 8.5% Isopropanol, 17% PEG 4000, 15% Glycerol, pH 7.5, VAPOR DIFFUSION, temperature 293K, pH 7.50 |
