2P4U
Crystal structure of acid phosphatase 1 (Acp1) from Mus musculus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.716, 168.539, 42.721 |
| Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xww |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.116 | 0.720 |
| Number of reflections | 48689 | |
| <I/σ(I)> | 19.09 | 1.7 |
| Completeness [%] | 98.7 | 98.4 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 10mM Sodium acetate, 70% MPD, VAPOR DIFFUSION, temperature 294K |
