2P3N
Thermotoga maritima IMPase TM1415
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-13 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.024, 103.873, 80.512 |
Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
Resolution | 42.000 - 2.200 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Homology built model from human AF and MJ IMPases |
RMSD bond length | 0.007 |
RMSD bond angle | 1.367 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.058 | 0.410 |
Number of reflections | 46203 | |
<I/σ(I)> | 14.1 | 2.1 |
Completeness [%] | 97.4 | 95.5 |
Redundancy | 3.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG 400, 0.2M magnesium sulfate, 50mM ammonium sulfate, 50mM cacodylate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |