2OY4
Uninhibited human MMP-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-27 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.294, 47.110, 61.325 |
| Unit cell angles | 77.73, 80.04, 77.01 |
Refinement procedure
| Resolution | 33.170 - 1.700 |
| R-factor | 0.23121 |
| Rwork | 0.225 |
| R-free | 0.29313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bzs |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.614 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.550 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.103 | 0.245 |
| Number of reflections | 30329 | |
| <I/σ(I)> | 4.7 | 2.6 |
| Completeness [%] | 90.3 | 88.3 |
| Redundancy | 1.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M Tris-HCl, 20% PEG3350, 200mM acetohydroxamic acid, 0.2M MgCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
