2OXT
Crystal structure of Meaban virus nucleoside-2'-O-methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 43 |
| Unit cell lengths | 82.977, 82.977, 170.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.850 - 2.900 |
| R-factor | 0.23444 |
| Rwork | 0.231 |
| R-free | 0.30597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r6a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.106 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.095 | 0.526 |
| Number of reflections | 24302 | |
| <I/σ(I)> | 12.4 | 3.1 |
| Completeness [%] | 95.4 | 96.8 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEGMME 2000, nickel chloride 0.01M, Tris 0.1 M , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
