2OXE
Structure of the Human Pancreatic Lipase-related Protein 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-19 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 216.922, 216.922, 123.618 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.880 - 2.800 |
R-factor | 0.22432 |
Rwork | 0.222 |
R-free | 0.26093 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bu8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.142 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 36137 | |
<I/σ(I)> | 12.7 | 2.76 |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 298 | 1.17M Ammonium sulfate, 0.1M Sodium cacodylate pH 5.5, 0.04M NaCl, cryoprotected with 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.50 |