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2OXC

Human DEAD-box RNA helicase DDX20, DEAD domain in complex with ADP

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-2
Synchrotron siteESRF
BeamlineID14-2
Temperature [K]100
Detector technologyCCD
Collection date2006-09-18
DetectorADSC QUANTUM 4
Wavelength(s)0.93300
Spacegroup nameP 31 2 1
Unit cell lengths63.800, 63.800, 214.300
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution38.460 - 1.300
R-factor0.15378
Rwork0.153
R-free0.17485
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1qva
RMSD bond length0.010
RMSD bond angle1.443
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareMOLREP
Refinement softwareREFMAC (5.3.0021)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0001.500
High resolution limit [Å]1.3001.300
Rmerge0.0570.458
Number of reflections125192
<I/σ(I)>23.55.2
Completeness [%]99.899.9
Redundancy109.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.227712% PEG3350, 200 mM Sodium chloride, 100 mM Bis-Tris, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K

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