2OXC
Human DEAD-box RNA helicase DDX20, DEAD domain in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93300 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 63.800, 63.800, 214.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.460 - 1.300 |
R-factor | 0.15378 |
Rwork | 0.153 |
R-free | 0.17485 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qva |
RMSD bond length | 0.010 |
RMSD bond angle | 1.443 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.500 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.057 | 0.458 |
Number of reflections | 125192 | |
<I/σ(I)> | 23.5 | 5.2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 10 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 277 | 12% PEG3350, 200 mM Sodium chloride, 100 mM Bis-Tris, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |