2OXC

Human DEAD-box RNA helicase DDX20, DEAD domain in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93300
Spacegroup name	P 31 2 1
Unit cell lengths	63.800, 63.800, 214.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.460 - 1.300
R-factor	0.15378
Rwork	0.153
R-free	0.17485
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qva
RMSD bond length	0.010
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.3.0021)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.500
High resolution limit [Å]	1.300	1.300
Rmerge	0.057	0.458
Number of reflections	125192
<I/σ(I)>	23.5	5.2
Completeness [%]	99.8	99.9
Redundancy	10	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	277	12% PEG3350, 200 mM Sodium chloride, 100 mM Bis-Tris, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K