2OX8

Human Scavenger Receptor C-type Lectin carbohydrate-recognition domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 32
Unit cell lengths	80.422, 80.422, 67.159
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.920 - 2.500
Rwork	0.233
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.900	2.570
High resolution limit [Å]	2.500	2.500
Number of reflections	16533
Completeness [%]	98.4	99.5
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	The protein solution contained 10mg ml-1 protein, 8mM CaCl2 , 8mM Tris pH=7.8, 20mM NaCl and 10mM Lewisx. The reservoir solution contained 8% Peg 8K, 0.2M ZnAc2 and 0.1 M Tris, pH 7.0. , VAPOR DIFFUSION, HANGING DROP, temperature 298K