2OW1

MMP-9 active site mutant with trifluoromethyl hydroxamate inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Detector technology	CCD
Collection date	2005-03-11
Detector	MAR CCD 165 mm
Spacegroup name	P 41 21 2
Unit cell lengths	55.720, 55.720, 260.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.790 - 2.200
R-factor	0.217
Rwork	0.217
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.180
High resolution limit [Å]	2.150	2.150
Rmerge	0.043	0.163
Number of reflections	19793
<I/σ(I)>	16.3	3.5
Completeness [%]	77.0	64.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1