2OV2
The crystal structure of the human RAC3 in complex with the CRIB domain of human p21-activated kinase 4 (PAK4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.96870 |
Spacegroup name | P 1 |
Unit cell lengths | 50.843, 73.859, 133.840 |
Unit cell angles | 88.58, 87.76, 70.64 |
Refinement procedure
Resolution | 62.110 - 2.100 |
R-factor | 0.1745 |
Rwork | 0.172 |
R-free | 0.22453 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g0n |
RMSD bond length | 0.011 |
RMSD bond angle | 1.485 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.110 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.147 | 0.602 |
Number of reflections | 106111 | |
<I/σ(I)> | 7.8 | 1.9 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M Ammonium acetate, 0.1M Citrate, 30% PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |