2OTW
Crystal structure of Fv polyglutamine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 165.290, 78.724, 49.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.25800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2gsg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.052 | 0.221 |
| Number of reflections | 27313 | |
| <I/σ(I)> | 22.8 | 2.9 |
| Completeness [%] | 97.2 | 81.8 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 298 | 0.1 M sodium citrate, 0.2 M NH4Ac, 27.5% PEG400, pH 6.5, EVAPORATION, temperature 298K |
