2OTV
Crystal structure of the complex formed between bovine trypsin and nicotinamide at 1.56 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 292 |
Detector technology | IMAGE PLATE |
Collection date | 2007-01-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54132 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.831, 58.579, 67.461 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.280 - 1.560 |
R-factor | 0.16883 |
Rwork | 0.168 |
R-free | 0.18586 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ayw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.313 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.280 | 1.600 |
High resolution limit [Å] | 1.560 | 1.560 |
Number of reflections | 29751 | |
Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | Ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |