2OTC
ORNITHINE TRANSCARBAMOYLASE COMPLEXED WITH N-(PHOSPHONACETYL)-L-ORNITHINE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-02-01 |
| Detector | SIEMENS |
| Spacegroup name | P 1 |
| Unit cell lengths | 104.030, 114.690, 93.780 |
| Unit cell angles | 86.99, 93.11, 118.81 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1otc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.480 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.262 |
| Number of reflections | 53778 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 58.0 | 20 |
| Redundancy | 1.8 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | PALO | 1 (mg/ml) | |
| 3 | 1 | drop | HEPES | 10 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 14 (%) | |
| 5 | 1 | reservoir | sodium acetate | 90 (mM) | |
| 6 | 1 | reservoir | 70 (mM) | ||
| 7 | 1 | reservoir | MPD | 2 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 45 (mM) |
