2ORB
The structure of the anti-c-myc antibody 9E10 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-25 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.593, 65.462, 117.689 |
| Unit cell angles | 90.00, 96.97, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| Rwork | 0.261 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2or9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 37773 | |
| <I/σ(I)> | 19.9 | 2.7 |
| Completeness [%] | 91.5 | 62.1 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.05 | 291 | 15% PEG 3350, 0.2M ammonium sulfate, 0.1 M sodium citrate buffer, pH 3.05, 0.02% sodium azide, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
