2OQ1
Tandem SH2 domains of ZAP-70 with 19-mer zeta1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.110, 63.370, 54.000 |
| Unit cell angles | 90.00, 114.44, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25500 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 23978 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 20%PEG 4000, 50mM sodium citrate,100 mM ammonium acetate, 20mM DTT, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
