2OOB
crystal structure of the UBA domain from Cbl-b ubiquitin ligase in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-20 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.608, 54.784, 94.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.150 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.25636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1UBQ 2ooa |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.753 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.041 | 0.128 |
| Number of reflections | 10568 | |
| <I/σ(I)> | 29.9 | 10.9 |
| Completeness [%] | 99.9 | 92.7 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.2M calcium chloride, 0.1M sodium acetate, 20% PEG6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
