2OO4

Structure of LNR-HD (Negative Regulatory Region) from human Notch 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	77.2
Detector technology	CCD
Collection date	2006-02-25
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	45.372, 74.706, 139.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.003
R-factor	0.229
Rwork	0.226
R-free	0.26800
Structure solution method	MIRAS
RMSD bond length	0.010
RMSD bond angle	1.174
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.003	2.003
Rmerge	0.075	0.405
Number of reflections	31791
<I/σ(I)>	15.5
Completeness [%]	97.2	98.1
Redundancy	4.9	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	298	100 mM BisTris, 200mM MgCl2, 18% PEG3350, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1		6.5	298	100 mM BisTris, 200mM MgCl2, 18% PEG3350, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1		6.5	298	100 mM BisTris, 200mM MgCl2, 18% PEG3350, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1		6.5	298	100 mM BisTris, 200mM MgCl2, 18% PEG3350, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K