2OLE
Crystal Structure Of Human Dipeptidyl Peptidase IV (DPPIV) Complex With Cyclic Hydrazine Derivatives
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2005-04-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.95078 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.858, 126.545, 112.137 |
| Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.22 |
| Rwork | 0.216 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 113734 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 81.6 | 93.8 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 24% PEG 4000, 0.1M Tris, 0.3M Sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
