2OL4
Crystal structure of plasmodium falciparum enoyl ACP reductase with triclosan reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 131.445, 131.445, 82.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 2.260 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1NHD |
| Data reduction software | XFIT |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 24626 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 66.6 | 99.6 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 2.35M (NH4)2SO4, 0.1M AcONa, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
