2OKR
Crystal Structure of the P38a-MAPKAP kinase 2 Heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2005-04-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 |
Unit cell lengths | 81.550, 81.550, 121.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.480 - 2.000 |
R-factor | 0.2385 |
Rwork | 0.239 |
R-free | 0.27210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lez |
RMSD bond length | 0.010 |
RMSD bond angle | 1.193 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.2SSI) |
Phasing software | MOLREP |
Refinement software | autoBUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.780 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.364 |
Total number of observations | 13657 | |
Number of reflections | 60104 | |
<I/σ(I)> | 9 | 2.2 |
Completeness [%] | 98.6 | 90.7 |
Redundancy | 3.06 | 2.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 25% Peg 3350, 250 mM Sodium Formate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |