2OKK

The X-ray crystal structure of the 65kDa isoform of Glutamic Acid Decarboxylase (GAD65)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	78.251, 99.057, 120.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.190 - 2.300
R-factor	0.2
Rwork	0.196
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2okj
RMSD bond length	0.009
RMSD bond angle	1.352
Data reduction software	HKL-2000
Data scaling software	SCALA (CCP4_3.2.17 23/9/2005)
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.190	54.640	2.420
High resolution limit [Å]	2.300	7.270	2.300
Total number of observations		2247	11442
Number of reflections	20717
Completeness [%]		91.86	98.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	20% ethanol, 100 mM MES, 10 mM 2-mercaptoethanol, 20 mM CaCl2, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K