2OKK
The X-ray crystal structure of the 65kDa isoform of Glutamic Acid Decarboxylase (GAD65)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 78.251, 99.057, 120.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.190 - 2.300 |
R-factor | 0.2 |
Rwork | 0.196 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2okj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.352 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA (CCP4_3.2.17 23/9/2005) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.190 | 54.640 | 2.420 |
High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
Total number of observations | 2247 | 11442 | |
Number of reflections | 20717 | ||
Completeness [%] | 91.86 | 98.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 20% ethanol, 100 mM MES, 10 mM 2-mercaptoethanol, 20 mM CaCl2, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |