2OJT
Structure and mechanism of kainate receptor modulation by anions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.92017 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.598, 97.732, 128.688 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.830 - 1.950 |
R-factor | 0.19223 |
Rwork | 0.190 |
R-free | 0.23291 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f34 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.331 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.048 | 0.380 |
Number of reflections | 44649 | |
<I/σ(I)> | 10.8 | 3.8 |
Completeness [%] | 97.9 | 99 |
Redundancy | 7.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 19% PEG 1000, 120 mM Br, 100 mM Tris-HCl, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |