2OID
Crystal structure of IRAK4 kinase domain complexed with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97943 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.116, 138.973, 89.198 |
| Unit cell angles | 90.00, 126.43, 90.00 |
Refinement procedure
| Resolution | 49.940 - 2.300 |
| R-factor | 0.24381 |
| Rwork | 0.241 |
| R-free | 0.29542 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2oib |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.366 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 63527 |
| <I/σ(I)> | 8.9 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.3M sodium malonate, 0.1M sodium acetate, 0.01M DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
