2OHG
Structural Basis for Glutamte Racemase Inhibition
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97956, 0.97969, 0.97189 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.581, 81.581, 100.237 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.880 - 2.500 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 | 0.234 |
| Number of reflections | 23929 | |
| <I/σ(I)> | 28.6 | 3.04 |
| Completeness [%] | 95.6 | 79.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 295 | 8% PEG6000, 7% Glycerol, 0.1M Bicine, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
