2OGV

Crystal Structure of the Autoinhibited Human c-Fms Kinase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.90000
Spacegroup name	P 21 21 2
Unit cell lengths	104.253, 57.298, 56.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.440 - 2.700
R-factor	0.20966
Rwork	0.206
R-free	0.27926
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rjb
RMSD bond length	0.007
RMSD bond angle	1.310
Data reduction software	HKL-2000
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.113
Number of reflections	9841
<I/σ(I)>	22.9	7.8
Redundancy	6.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	30% PEG 8000, 0.2M Ammonium sulfate, 0.1M MES pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K