2OGO
The crystal structure of the large ribosomal subunit from Deinococcus radiodurans complexed with the pleuromutilin derivative retapamulin (SB-275833)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979290 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 170.115, 405.873, 695.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.960 - 3.660 |
Rwork | 0.260 |
R-free | 0.33400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.790 |
High resolution limit [Å] | 3.660 | 3.660 |
Rmerge | 0.190 | 0.791 |
Number of reflections | 243598 | |
<I/σ(I)> | 9.1 | 1.4 |
Completeness [%] | 93.1 | 70 |
Redundancy | 8.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | Ethanol, Dimethylhexanediol, MgCl2, HEPES, NH4Cl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ethanol | ||
10 | 1 | 2 | HEPES | ||
11 | 1 | 2 | NH4Cl | ||
2 | 1 | 1 | Dimethylhexanediol | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | HEPES | ||
5 | 1 | 1 | NH4Cl | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | Ethanol | ||
8 | 1 | 2 | Dimethylhexanediol | ||
9 | 1 | 2 | MgCl2 |