2OGE
x-ray structure of S. venezuelae DesV in its internal aldimine form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-08 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.798, 143.820, 81.132 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 30.500 - 2.050 |
| R-factor | 0.162 |
| Rwork | 0.159 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.150 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 92487 | |
| <I/σ(I)> | 15.5 | 4.6 |
| Completeness [%] | 92.0 | 87.3 |
| Redundancy | 6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 6 | 298 | 12% PEG 3400, 1% ethylene glycol, 50 mM MES, 50 mM glutamate, 75 mM NaCl, pH 6.0, batch, temperature 298K |
