2OG4

Structure of an expanded Jab1-MPN-like domain of splicing factor Prp8p from yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-04
Detector	MAR CCD 165 mm
Spacegroup name	P 42 21 2
Unit cell lengths	78.465, 78.465, 122.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.20382
Rwork	0.202
R-free	0.24149
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.186
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	26084
<I/σ(I)>	16.1	2.1
Completeness [%]	97.8	96
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2.5 % PEG 3350, 0.01 M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2.5 % PEG 3350, 0.01 M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2.5 % PEG 3350, 0.01 M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K