2OF5

Oligomeric Death Domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	110
Detector technology	CCD
Collection date	2006-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0080
Spacegroup name	P 65
Unit cell lengths	138.400, 138.400, 207.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.200
Rwork	0.236
R-free	0.27500
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	3.200	3.200
Rmerge	0.080	0.110
Number of reflections	28927
<I/σ(I)>	15	2.1
Completeness [%]	75.0	95
Redundancy	10.3	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	5.5% PEG 3350, 200mM NaCl and 100mM Na/K phosphate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	5.5% PEG 3350, 200mM NaCl and 100mM Na/K phosphate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K