2OE1
Crystal Structure of Mitochondrial Thioredoxin 3 from Saccharomyces cerevisiae (reduced form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-12-31 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54179 |
Spacegroup name | P 31 |
Unit cell lengths | 49.575, 49.575, 94.554 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.410 - 2.100 |
R-factor | 0.16258 |
Rwork | 0.160 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eru |
RMSD bond length | 0.017 |
RMSD bond angle | 1.565 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.410 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.111 | 0.393 |
Number of reflections | 14192 | |
<I/σ(I)> | 14.4 | 3.6 |
Completeness [%] | 93.5 | 91.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.2M ammonium sulfate, 0.1M sodium acetate, 8% PEG 4000, 15% glycerol, 8mM DTT, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |