2ODJ
Crystal structure of the outer membrane protein OprD from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97922 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 111.370, 97.525, 95.788 |
Unit cell angles | 90.00, 119.63, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.900 |
R-factor | 0.2476 |
Rwork | 0.248 |
R-free | 0.31540 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.143 | 0.736 |
Number of reflections | 22385 | |
<I/σ(I)> | 7 | 2.8 |
Completeness [%] | 98.2 | 82.5 |
Redundancy | 7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4 | 295 | 0.1 M LiSO4, 0.1 M NaCl, 0.1 M Na citrate, 28-32% PEG 400 (pH 3.5), pH 4.0, VAPOR DIFFUSION, temperature 295K |