2OAN
Structure of oxidized beta-actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.174, 222.589, 133.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.980 - 2.606 |
| R-factor | 0.21383 |
| Rwork | 0.210 |
| R-free | 0.28812 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j6z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.389 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.980 | 2.680 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.129 | 0.646 |
| Number of reflections | 52031 | |
| <I/σ(I)> | 9.34 | 1.72 |
| Completeness [%] | 94.7 | 76.4 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.6 M (NH4)2SO4, 0.1 M HEPES, 0.1 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
