2OA8
Crystal Structure of mTREX1 with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-21 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.850, 57.141, 68.470 |
| Unit cell angles | 90.00, 107.47, 90.00 |
Refinement procedure
| Resolution | 61.900 - 2.100 |
| R-factor | 0.19514 |
| Rwork | 0.192 |
| R-free | 0.25916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ioc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.952 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.860 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.110 |
| Rmerge | 0.050 | 0.231 |
| Number of reflections | 37899 | |
| <I/σ(I)> | 11.2 | 4.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.51 | 3.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22% PEG 3350, 50mM MES, pH 6.5, 50mM HEPES, pH 7.15, 2mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 10 | 1 | 2 | HOH | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | MES | ||
| 8 | 1 | 2 | HEPES | ||
| 9 | 1 | 2 | CaCl2 |
