2O93
Crystal structure of NFAT bound to the HIV-1 LTR tandem kappaB enhancer element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-14 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.691, 95.299, 159.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.050 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p7h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 6.550 | 3.050 |
| Rmerge | 0.129 | 0.050 | 0.538 |
| Number of reflections | 26944 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 97.9 | 99.8 | 83.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 50mM Hepes pH 7.0, 15mM Magnesium Acetate, 250mM Ammonium Acetate, 7.5% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Ammonium Acetate | ||
| 3 | 1 | 1 | Magnesium Acetate | ||
| 4 | 1 | 1 | Hepes | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | Ammonium Acetate | ||
| 7 | 1 | 2 | Magnesium Acetate |
