2O8X
Crystal structure of the "-35 element" promoter recognition domain of Mycobacterium tuberculosis SigC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 161.330, 161.330, 161.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.010 - 3.000 |
| R-factor | 0.21628 |
| Rwork | 0.214 |
| R-free | 0.27131 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1or7 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.235 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.290 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 7040 | |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.8M ammonium sulphate, 0.1M MES (pH 6.5), 1mM DTT, 5% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
