2O8M
Crystal structure of the S139A mutant of Hepatitis C Virus NS3/4A protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-01 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 224.640, 224.640, 75.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.194 |
R-free | 0.22800 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BUSTER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.042 | 0.572 |
Number of reflections | 46014 | |
<I/σ(I)> | 19.4 | 1.1 |
Completeness [%] | 94.1 | 58.7 |
Redundancy | 3 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.25 to 1.5M NaCl, 0.1 M MES, .1M Na/K PO4, 5mM beta-mercaptoethanol, pH 5.6-6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |