2O7Q

Crystal Structure of the A. thaliana DHQ-dehydroshikimate-SDH-shikimate-NADP(H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	200
Wavelength(s)	0.9807
Spacegroup name	P 32 2 1
Unit cell lengths	96.611, 96.611, 115.945
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.2593
R-free	0.21380
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.770
Number of reflections	51079
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	0.4M potassium sodium tartrate, 2.4M ammonium sulfate, 0.1M tri-sodium citrate dihydrate (pH5.6), 1mM shikimate, 50 mM dehydroquinate soaked in, VAPOR DIFFUSION, HANGING DROP, temperature 295K