2O6R

Structural diversity of the hagfish Variable Lymphocyte Receptors B61

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	295
Detector technology	CCD
Collection date	2006-06-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97175
Spacegroup name	P 21 21 21
Unit cell lengths	42.774, 55.355, 81.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.340 - 2.300
R-factor	0.191
Rwork	0.191
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m10
RMSD bond length	0.012
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300	2.300
Number of reflections	8477
<I/σ(I)>	63.2	46.6
Completeness [%]	93.6	68.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	30% PEG 4000, 0.1M Tris-Hcl, 0.2M sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K