2O6Q

Structural diversity of the hagfish Variable Lymphocyte Receptors A29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	298
Detector technology	CCD
Collection date	2006-06-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97175
Spacegroup name	P 32 2 1
Unit cell lengths	95.226, 95.226, 77.556
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.930 - 2.500
R-factor	0.227
Rwork	0.227
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ozn
RMSD bond length	0.020
RMSD bond angle	2.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500	2.500
Number of reflections	13115
<I/σ(I)>	17.4	2.3
Completeness [%]	91.0	61.5
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	33% PEG 1000, 0.1M Bis-Tris, 0.2M magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K