2O6G
Crystal structure of IRF-3 bound to the interferon-b enhancer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.020, 105.320, 353.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| Rwork | 0.251 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.238 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.106 | 0.033 | 0.317 |
| Number of reflections | 27180 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 87.0 | 83.3 | 87.9 |
| Redundancy | 5.4 | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 100 mM Tris, 20% (w/v) PEG 6000, 500 mM NH4OAc, 100 mM NaCl, 5 mM MgCl2, 5% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris | ||
| 10 | 1 | 2 | NH4OAc | ||
| 11 | 1 | 2 | NaCl | ||
| 12 | 1 | 2 | MgCl2 | ||
| 13 | 1 | 2 | HOH | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | NH4OAc | ||
| 4 | 1 | 1 | NaCl | ||
| 5 | 1 | 1 | MgCl2 | ||
| 6 | 1 | 1 | glycerol | ||
| 7 | 1 | 1 | HOH | ||
| 8 | 1 | 2 | Tris | ||
| 9 | 1 | 2 | PEG 6000 |
